Project Information

PI nameRegina Kemunto Mayaka
Project titleCHEMICAL CHARACTERISATION AND ANTIMICROBIAL ACTIVITY OF COMPOUNDS FROM SOME SELECTED MEDICINAL KENYAN GANODERMA AND TRAMETES SPECIES
PartnersNational Research Fund
Collaborators
  • Prof. J.O Omolo
Grant amount810,000
Project StatusEnded
Project LocationNjoro, Egerton
Project descriptionPolypores are a group of tough, leathery poroid mushrooms typically lacking the distinct stalk. They are a fascinating group of fungi, although they are usually ignored by most mycophiles because of their typical inedibility, unfamiliar habitat and general obscurity. Beyond their traditional use in folk medicine, contemporary research has suggested many applications of polypores for treatment of illness related to the immune system and cancer. The main objective was to characterise antimicrobial compounds from some selected medicinal Kenyan Ganoderma and Trametes species collected from Mau, Kericho area, Kabarnet and Kerio valley forests in Kenya. The dried, ground fruiting bodies of Ganoderma adspersum, Ganoderma applanatum and Ganoderma australe and Trametes elegans were extracted with methanol to obtain a methanol crude extract, then successively extracted using ethyl acetate solvent to obtain an ethyl acetate extract. The crude extracts were fractionated and purified using column chromatography technique and further purification of some fractions was achieved on sephadex LH20.. The chemical structures were determined on the basis of NMR spectroscopic data from 1H and 13C NMR, HSQC, HMBC, 1H-1H COSY, and NOESY experiments.
Project Start01/04/2014
Project End01/04/2019
Expected outputsThree peer reviewed journals and a doctorate degree in Chemistry